DYNC1LI2 Polyklonaler Antikörper

DYNC1LI2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18885-1-AP

Synonyme

DNCLI2, DYNC1LI2, LIC 2, LIC2, LIC53/55

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 18885-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18885-1-AP (DYNC1LI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

18885-1-AP bindet in WB, ELISA DYNC1LI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYNC1LI2 fusion protein Ag13452
• Vollständiger Name: dynein, cytoplasmic 1, light intermediate chain 2
• Berechnetes Molekulargewicht: 492 aa, 54 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC025959
• Gene symbol: DYNC1LI2
• Gene ID (NCBI): 1783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytoplasmic dynein is a microtubule-associated motor protein. DYNC1LI2 (Cytoplasmic dynein 1 light intermediate chain 2), also known as LIC2, belongs to the dynein light intermediate chain family. DYNC1LI2 acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. It may play a role in binding dynein to membranous organelles or chromosomes. Northern blot analysis of rat tissues detected high expression of DYNC1LI2 in skeletal muscle, testis, heart, lung, and brain, and lower expression in liver, kidney, and spleen (PMID: 7738094).

Publikationen

Species	Application	Title
mouse	WB	PLoS Genet; Dync1li1 is required for the survival of mammalian cochlear hair cells by regulating the transportation of autophagosomes.; Authors - Yuan Zhang
human	WB	Cell Cycle; Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.; Authors - Ankur A Gholkar
mouse	WB	Nature; A male germ-cell-specific ribosome controls male fertility; Authors - Huiling Li