DTX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DTX1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 18350-1-AP

Synonyme

deltex homolog 1 (Drosophila), Deltex1, DTX1, hDTX1, hDx 1, Protein deltex 1

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Galerie der Validierungsdaten

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  WB analysis using 18350-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18350-1-AP (DTX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 18350-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 18350-1-AP (DTX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of mouse olfactory epithelium tissue using 18350-1-AP

  Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of DTX1 antibody (18350-1-AP). Basal cell staining as expected with Sus cell staining on the apical layer. By Dr. Brian Lin (Schwob Lab).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Riechepithelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

18350-1-AP bindet in WB, IHC, IF, ELISA DTX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DTX1 fusion protein Ag13172
• Vollständiger Name: deltex homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 620 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC048216
• Gene symbol: DTX1
• Gene ID (NCBI): 1840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Deltex homolog 1 (E3 ubiquitin-protein ligase DTX1) is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.DTX1 is conserved from Drosophila, and is expressed in a wide array of tissues, particularly in the central nervous system, testes, and endothelial cells, mainly colocalized with Notch1.It contains a RING-type zinc finger and 2 WWE domains.

Publikationen

Species	Application	Title
human	WB	Virus Res; Gambogic acid inhibits HBx-mediated hepatitis B virus replication by targeting the DTX1-Notch signaling pathway; Authors - Xu Wen ; KD Validated
human	WB	Cancer Sci; Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.; Authors - Xin-Yu Ding