DSPP Polyklonaler Antikörper

DSPP Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 55396-1-AP

Synonyme

Dentin phosphophoryn, dentin sialophosphoprotein, DFNA39, DGI1, DMP3, DPP, DPP Dentin sialoprotein, DSP, DSPP, DTDP2

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Galerie der Validierungsdaten

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  WB analysis using 55396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55396-1-AP (DSPP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

55396-1-AP bindet in WB, IF, ELISA DSPP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dentin sialophosphoprotein
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_014208
• Gene symbol: DSPP
• Gene ID (NCBI): 1834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. This antibody recognizes the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).

Publikationen

Species	Application	Title
human	WB	Int J Nanomedicine; Multifunctional Lithium-Doped Mesoporous Nanoparticles for Effective Dentin Regeneration in vivo; Authors - Zitian Liang
human	WB	Int Endod J; Effects of phase-transited lysozyme on adhesion, migration and odontogenic differentiation of human dental pulp cells: An in vitro study; Authors - Jiange Zhao