DSG1 Polyklonaler Antikörper

DSG1 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 24587-1-AP

Synonyme

Cadherin family member 4, CDHF4, desmoglein 1, Desmosomal glycoprotein 1, DG1, DGI, DSG, DSG1, Pemphigus foliaceus antigen, PPKS1, SPPK1

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Galerie der Validierungsdaten

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  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 24587-1-AP

  Immunofluorescent analysis of A431 cells using 24587-1-AP (DSG1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Produktinformation

24587-1-AP bindet in WB, IHC, IF, ELISA DSG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DSG1 fusion protein Ag20184
• Vollständiger Name: desmoglein 1
• Berechnetes Molekulargewicht: 1049 aa, 114 kDa
• GenBank-Zugangsnummer: BC153001
• Gene symbol: DSG1
• Gene ID (NCBI): 1828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein that is highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and has a crucial role in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).

Publikationen

Species	Application	Title
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
human	IF	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
mouse	WB,IF	Microscopy (Oxf); Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.; Authors - Shoko Ishikawa
mouse	IF	Stem Cell Reports; Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development; Authors - Florian Hermans
human	IHC,IF	Sci Rep; Senescence-related deterioration of intercellular junctions in the peritoneal mesothelium promotes the transmesothelial invasion of ovarian cancer cells.; Authors - Martyna Pakuła