DRG2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DRG2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14743-1-AP

Synonyme

DRG 2, DRG2

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Galerie der Validierungsdaten

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  WB analysis using 14743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14743-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14743-1-AP

  IP Result of anti-DRG2 (IP:14743-1-AP, 4ug; Detection:14743-1-AP 1:2000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human kidney using 14743-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14743-1-AP (DRG2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14743-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14743-1-AP (DRG2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 14743-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14743-1-AP (DRG2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushirngewebe, SMMC-7721-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

14743-1-AP bindet in WB, IP, IHC, IF, ELISA DRG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DRG2 fusion protein Ag6433
• Vollständiger Name: developmentally regulated GTP binding protein 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41-45 kDa
• GenBank-Zugangsnummer: BC000493
• Gene symbol: DRG2
• Gene ID (NCBI): 1819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. DRG2 plays a critical role in control of the cell cycle and apoptosis (PMID:27669826, PMID:14759600). DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.

Publikationen

Species	Application	Title
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
human	WB	Int J Mol Sci; DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial Dysfunction.; Authors - Anh-Nhung Le ; KO Validated
human	WB,IF,IP	Oxid Med Cell Longev; DRG2 Accelerates Senescence via Negative Regulation of SIRT1 in Human Diploid Fibroblasts; Authors - Bing Si Li ; KD Validated
mouse	IF	FASEB J; Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain.; Authors - Rebecca Chase
human,monkey	WB	Mol Cell Endocrinol; RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation.; Authors - Gr?tsch Helga H
human	WB	Biochem Biophys Res Commun; Developmentally regulated GTP-binding protein 2 depletion leads to mitochondrial dysfunction through downregulation of dynamin-related protein 1.; Authors - Mai-Tram Vo ; KD Validated