DPP4/CD26 Polyklonaler Antikörper

DPP4/CD26 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 29403-1-AP

Synonyme

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

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Galerie der Validierungsdaten

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  WB analysis using 29403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29403-1-AP (DPP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HT-29 using 29403-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human DPP4/CD26 (29403-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HT-29-Zellen
• Erfolgreiche Detektion in FC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

29403-1-AP bindet in WB, FC, ELISA DPP4/CD26 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DPP4/CD26 fusion protein Ag30160
• Vollständiger Name: dipeptidyl-peptidase 4
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC013329
• Gene symbol: DPP4
• Gene ID (NCBI): 1803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).

Publikationen

Species	Application	Title
mouse	WB	Front Allergy; High-fat diet and palmitic acid amplify airway type 2 inflammation; Authors - Kris Genelyn Dimasuay
mouse	WB	Biomed Pharmacother; Fructus Lycii and Salvia miltiorrhiza Bunge extract attenuate oxidative stress-induced photoreceptor ferroptosis in retinitis pigmentosa; Authors - Yijing Yang
human	WB	Anal Chem; Ultrasensitive Fluorescent Probes Reveal an Adverse Action of Dipeptide Peptidase IV and Fibroblast Activation Protein during Proliferation of Cancer Cells; Authors - Qiuyu Gong