DPEP1 Monoklonaler Antikörper

DPEP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B4F4

Kat-Nr. : 68081-1-Ig

Synonyme

Dehydropeptidase I, Dipeptidase 1, dipeptidase 1 (renal), DPEP1, hRDP, MBD1, MDP, Microsomal dipeptidase, RDP, Renal dipeptidase

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Galerie der Validierungsdaten

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  WB analysis of pig pancreas using 68081-1-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 68081-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit kidney using 68081-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 68081-1-Ig

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68081-1-Ig

  WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells.

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  IHC staining of human kidney using 68081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 68081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 68081-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Hausschwein-Pankreasgewebe, HepG2-Zellen, Kaninchennierengewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68081-1-Ig bindet in WB, IHC, IF, ELISA DPEP1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DPEP1 fusion protein Ag14591
• Vollständiger Name: dipeptidase 1 (renal)
• Berechnetes Molekulargewicht: 411 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC017023
• Gene symbol: DPEP1
• Gene ID (NCBI): 1800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.