DOCK5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DOCK5 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29938-1-AP

Synonyme

dedicator of cytokinesis 5, DKFZp451J181, DKFZp779M164, DKFZp781J211, DOCK5

---

Galerie der Validierungsdaten

• 

  WB analysis using 29938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 29938-1-AP

  WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells.

• 

  IP experiment of DU 145 using 29938-1-AP

  IP result of anti-DOCK5(IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug.

• 

  IF Staining of U2OS using 29938-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A549-Zellen, DU 145-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, NIH3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: DU 145-Zellen
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29938-1-AP bindet in WB, IP, IF, ELISA DOCK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOCK5 fusion protein Ag32193
• Vollständiger Name: dedicator of cytokinesis 5
• Berechnetes Molekulargewicht: 1870 aa, 215 kDa
• Beobachtetes Molekulargewicht: 190 kDa
• GenBank-Zugangsnummer: BC137175
• Gene symbol: DOCK5
• Gene ID (NCBI): 80005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.