DOCK3; MOCA Polyklonaler Antikörper

DOCK3; MOCA Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20683-1-AP

Synonyme

dedicator of cytokinesis 3, DOCK3, DOCK3; MOCA, KIAA0299, MOCA, Modifier of cell adhesion, PBP, Presenilin binding protein

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Galerie der Validierungsdaten

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  WB analysis using 20683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20683-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20683-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20683-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20683-1-AP bindet in WB, IHC, ELISA DOCK3; MOCA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dedicator of cytokinesis 3
• Berechnetes Molekulargewicht: 233 kDa
• Beobachtetes Molekulargewicht: 233 kDa
• GenBank-Zugangsnummer: NM_004947
• Gene symbol: DOCK3
• Gene ID (NCBI): 1795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dedicator of cytokinesis 3 (DOCK3), also named as MOCA and PBP , is a ~180 kDa protein involved in signaling trasduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3β (GSK-3β) at the plasma membrane, thereby promoteing axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS.

Publikationen

Species	Application	Title
mouse	WB	Diabetes; Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.; Authors - Hua Qu
human	WB	Int J Biochem Cell Biol; Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer.; Authors - Xingli Zhu