DNMT3B Polyklonaler Antikörper

DNMT3B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 26971-1-AP

Synonyme

DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, DNMT3B, ICF, M.HsaIIIB

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Galerie der Validierungsdaten

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  WB analysis using 26971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Mammakarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

26971-1-AP bindet in WB, IHC, IF, CoIP, ChIP, ELISA DNMT3B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNMT3B fusion protein Ag25117
• Vollständiger Name: DNA (cytosine-5-)-methyltransferase 3 beta
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 85-96 kDa
• GenBank-Zugangsnummer: NM_001207055
• Gene symbol: DNMT3B
• Gene ID (NCBI): 1789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.

Publikationen

Species	Application	Title
human,mouse	IF,WB	Protein Cell; The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting; Authors - Yuhan Chen
human	WB	Front Cell Dev Biol; miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis; Authors - Dong Pan
rat	IHC	Andrology; Methylation of eNOS in the rat penile corpus cavernosum under different pathological states and its relationship with erectile function; Authors - Na Wang
human	WB	Brain Sci; Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes.; Authors - Kalaiselvi Sivalingam
human	WB	Biol Res; DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.; Authors - Ying Zhu
human	WB	Integr Cancer Ther; Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.; Authors - Liang Wang