DNM3 Polyklonaler Antikörper

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• KD/KO Validated

DNM3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14737-1-AP

Synonyme

DNM3, dynamin 3, Dynamin, testicular, KIAA0820, T dynamin

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Galerie der Validierungsdaten

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  WB analysis using 14737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14737-1-AP (DNM3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14737-1-AP

  IP result of anti-DNM3(IP:14737-1-AP, 4ug; Detection:14737-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of mouse brain using 14737-1-AP

  IP Result of anti-DNM3 (IP:14737-1-AP, 4ug; Detection:14737-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human gliomas using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14737-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM3 antibody (14737-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

14737-1-AP bindet in WB, IP, IHC, IF, ELISA DNM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNM3 fusion protein Ag6381
• Vollständiger Name: dynamin 3
• Berechnetes Molekulargewicht: 98 kDa
• Beobachtetes Molekulargewicht: 97-100 kDa
• GenBank-Zugangsnummer: BC064546
• Gene symbol: DNM3
• Gene ID (NCBI): 26052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)

Publikationen

Species	Application	Title
	IF	Mol Cell Proteomics; Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals; Authors - Brett Nixon
mouse	IF	Mol Cell Proteomics; Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals.; Authors - Brett Nixon
mouse	WB,IHC,IF	J Biol Chem; Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.; Authors - Reid Andrew T AT
human	IF	Mol Hum Reprod; Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis.; Authors - Wei Zhou
human	WB	Clin Exp Metastasis; Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines.; Authors - Natascia Marino ; KD Validated
mouse	WB,IF	Biol Reprod; Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis.; Authors - Wei Zhou