DNAJB12 Polyklonaler Antikörper

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• KD/KO Validated

DNAJB12 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 16780-1-AP

Synonyme

DJ10, DNAJB12

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Galerie der Validierungsdaten

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  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16780-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16780-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16780-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16780-1-AP

  IP Result of anti-DNAJB12 (IP:16780-1-AP, 4ug; Detection:16780-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, humanes Hirngewebe, humanes Lebergewebe, humanes Nierengewebe, MCF-7-Zellen, MKN-45-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

16780-1-AP bindet in WB, IP, IF, ChIP, ELISA DNAJB12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB12 fusion protein Ag10193
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 12
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC011812
• Gene symbol: DNAJB12
• Gene ID (NCBI): 54788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,ChIP	Mol Cell; An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.; Authors - Jin Zhou
human	IP	Nat Commun; The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes.; Authors - Erna Davydova
	WB, IP	J Cell Biol; Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.; Authors - Yu-Jie Chen
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
human	WB,IF	J Cell Biol; Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication; Authors - Jeffrey M Williams
human	IF	J Virol; The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.; Authors - Parikshit Bagchi