DNA2 Polyklonaler Antikörper

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• KD/KO Validated

DNA2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 18727-1-AP

Synonyme

DNA2, DNA2L, KIAA0083

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 18727-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18727-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 18727-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 18727-1-AP

  IP result of anti-DNA2 (IP:18727-1-AP, 4ug; Detection:18727-1-AP 1:300) with mouse liver tissue lysate 4800 ug.

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  IHC staining of human cervical cancer using 18727-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18727-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18727-1-AP

  Immunofluorescent analysis of HepG2 cells using 18727-1-AP (DNA2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18727-1-AP bindet in WB, IP, IHC, IF, ELISA DNA2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: DNA replication helicase 2 homolog (yeast)
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: NM_001080449
• Gene symbol: DNA2
• Gene ID (NCBI): 1763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.

Publikationen

Species	Application	Title
human	WB,IF	Nat Cell Biol; Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.; Authors - Ahmed Emam ; KD Validated
mouse	WB	Genes Dev; Abro1 maintains genome stability and limits replication stress by protecting replication fork stability.; Authors - Shengfeng Xu ; KO Validated
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
human	WB	Cell Cycle; The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining.; Authors - Feng-Ling Tsai ; KD Validated
human	WB	Cells; Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.; Authors - Huaping Xiao ; KD Validated
human	IHC	Front Mol Biosci; Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.; Authors - Yongqu Lu