DMAP1 Monoklonaler Antikörper

DMAP1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4E8

Kat-Nr. : CL488-67183

Synonyme

DKFZp686L09142, DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425, SWC4

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Galerie der Validierungsdaten

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  IF Staining of HEK-293 using CL488-67183

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 DMAP1 antibody (CL488-67183, Clone: 1A4E8 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-67183

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DMAP1 (CL488-67183, Clone:1A4E8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67183 bindet in IF, FC (Intra) DMAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DMAP1 fusion protein Ag28906
• Vollständiger Name: DNA methyltransferase 1 associated protein 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC002855
• Gene symbol: DMAP1
• Gene ID (NCBI): 55929
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).