DLD Monoklonaler Antikörper

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• KD/KO Validated

DLD Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2E7G5

Kat-Nr. : 67702-1-Ig

Synonyme

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

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Galerie der Validierungsdaten

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  WB analysis using 67702-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67702-1-Ig

  WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells.

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  WB analysis of NIH/3T3 using 67702-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67702-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67702-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67702-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

67702-1-Ig bindet in WB, IHC, ELISA DLD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DLD fusion protein Ag9680
• Vollständiger Name: dihydrolipoamide dehydrogenase
• Berechnetes Molekulargewicht: 509 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC018696
• Gene symbol: DLD
• Gene ID (NCBI): 1738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Publikationen

Species	Application	Title
human	IHC	Front Immunol; The signature of cuproptosis-related immune genes predicts the tumor microenvironment and prognosis of prostate adenocarcinoma; Authors - Kai Yao