DLD Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DLD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Rezensionen (1)

Kat-Nr. : 16431-1-AP

Synonyme

DLDH, EC:1.8.1.4, GCSL, LAD, PHE3

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  WB analysis of HeLa using 16431-1-AP

  WB result of DLD antibody (16431-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells.

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  WB analysis using 16431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16431-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16431-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16431-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16431-1-AP

  IP result of anti-DLD (IP:16431-1-AP, 4ug; Detection:16431-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16431-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLD antibody (16431-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 16431-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLD antibody (16431-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Lebergewebe, L02-Zellen, Mausherzgewebe, Mauslebergewebe, Rattenherzgewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:500000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 31 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16431-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA DLD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DLD fusion protein Ag9729
• Vollständiger Name: dihydrolipoamide dehydrogenase
• Berechnetes Molekulargewicht: 509 aa, 54 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC018696
• Gene symbol: DLD
• Gene ID (NCBI): 1738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
		Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors - ; KO Validated
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia ; KD Validated
human	WB	Redox Biol; Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.; Authors - Yingying Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (07-12-2023)

Great antibody for WB. Very strong signal and very clean background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse liver

DLD Antibody Western Blot validation (1:4000 dilution) in Mouse liver (Cat no:16431-1-AP)