DIAPH3 Polyklonaler Antikörper

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• KD/KO Validated

DIAPH3 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-14342

Synonyme

diap3, DIAPH3, Diaphanous related formin 3, DKFZp434C0931, DKFZp686A13178, DRF3, FLJ34705, mDia2, Protein diaphanous homolog 3

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL488-14342

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DIAPH3 antibody (CL488-14342) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-14342 bindet in IF DIAPH3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIAPH3 fusion protein Ag5656
• Vollständiger Name: diaphanous homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 125-137 kDa
• GenBank-Zugangsnummer: BC048963
• Gene symbol: DIAPH3
• Gene ID (NCBI): 81624
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.