DIAPH3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DIAPH3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 14342-1-AP

Synonyme

diap3, DIAPH3, Diaphanous related formin 3, DRF3, mDia2, Protein diaphanous homolog 3

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 14342-1-AP

  WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.

• 

  WB analysis of HeLa using 14342-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14342-1-AP

  WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells.

• 

  WB analysis of DU 145 using 14342-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14342-1-AP

  IP Result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human cervical cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse testis using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IHC staining of human cervical cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 14342-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14342-1-AP

  Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, DU 145-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Prostatakarzinomgewebe, humanes Zervixkarzinomgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

14342-1-AP bindet in WB, IP, IHC, IF, ELISA DIAPH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIAPH3 fusion protein Ag5656
• Vollständiger Name: diaphanous homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 125-137 kDa
• GenBank-Zugangsnummer: BC048963
• Gene symbol: DIAPH3
• Gene ID (NCBI): 81624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

Publikationen

Species	Application	Title
mouse,human	IP	Blood; Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.; Authors - Yijie Liu ; KO Validated
human	IF	Sci Adv; Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice.; Authors - Amar J Majmundar
human	IHC,WB	Aging (Albany NY); The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer; Authors - Xiaowei Chen
human	WB	Cell Res; Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.; Authors - Stastna Jana J ; KD Validated
human	WB,IHC,IF	EMBO J; TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.; Authors - Qichao Huang
human	WB	Elife; Generation of stress fibers through myosin-driven reorganization of the actin cortex; Authors - Jaakko I Lehtimäki ; KD Validated