DHX37 Polyklonaler Antikörper

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• KD/KO Validated

DHX37 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 29741-1-AP

Synonyme

DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517

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Galerie der Validierungsdaten

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  WB analysis using 29741-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29741-1-AP (DHX37 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 29741-1-AP

  WB result of DHX37 antibody (29741-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX37 transfected HEK-293 cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29741-1-AP bindet in WB, ELISA DHX37 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX37 fusion protein Ag29909
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 37
• Berechnetes Molekulargewicht: 1157 aa, 130 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC037964
• Gene symbol: DHX37
• Gene ID (NCBI): 57647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHX37, also known as DDX37, KIAA1517. It is an ATP-bound RNA helicase that plays a role in the maturation of small ribosomal subunits in ribosomal biogenesis (PMID:30582406). The presence of DHX37 in early pre-ribosomal particles was monitored by a quality control pathway, and failure to recruit DHX37 resulted in pre-RRNA degradation. DHX37 binds directly to U3 small nucleolar RNA (snoRNA), and the catalytic activity of the helicase is required for dissociation of U3 small nucleolar RNA from the pre-ribosomal complex.In addition, it plays a role in early testicular development (PMID:31287541, PMID:31337883). It may also have an effect on brain development (PubMed:31256877). It is expressed in the fallopian tubes, ovaries, uterus, testes and brain (PMID: 31256877,PMID: 31337883).

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); DHX37 Is a Promising Prognostic Biomarker and a Therapeutic Target for Immunotherapy and Chemotherapy in HCC; Authors - Nanbin Liu