DHX32 Polyklonaler Antikörper

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• KD/KO Validated

DHX32 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 19808-1-AP

Synonyme

DDX32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH box protein 32, DHLP1, DHX32, HuDDX32

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 19808-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 19808-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19808-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 19808-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19808-1-AP (DHX32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19808-1-AP (DHX32 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A431-Zellen, COLO 320-Zellen, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

19808-1-AP bindet in WB, IHC, ELISA DHX32 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX32 fusion protein Ag13834
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 32
• Berechnetes Molekulargewicht: 743 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa, 75 kDa
• GenBank-Zugangsnummer: BC002473
• Gene symbol: DHX32
• Gene ID (NCBI): 55760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHX32, a new member of the DEAH subfamily of DEAD box proteins, is a novel putative RNA helicase with an activation-dependent paten of expression and characterized by a conserved helicase domain consisting of 7 to 8 conserved motifs. RNA helicases of the DEAD box family are highly conserved enzymes that are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DHX32 may play an important role in the development of colorectal cancer. This is a rabbit polyclonal anti-DHX32 antibody raised against part of the C-terminal chain of human DHX32.

Publikationen

Species	Application	Title
human	WB,IHC	Oncol Lett; DHX32 expression is an indicator of poor breast cancer prognosis.; Authors - Meng Wang
human	WB,IHC	Transl Cancer Res; Silencing of DHX32 increases the proliferation of liver cancer cells.; Authors - Min-Jing Cai ; KD Validated