DHRS3 Polyklonaler Antikörper

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• KD/KO Validated

DHRS3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 15393-1-AP

Synonyme

DD83.1, DHRS3, RDH17, retSDR1, Rsdr1, SDR1, SDR16C1

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Galerie der Validierungsdaten

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  WB analysis using 15393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15393-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 15393-1-AP

  IP Result of anti-DHRS3 (IP:15393-1-AP, 4ug; Detection:15393-1-AP 1:500) with A375 cells lysate 3600ug.

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  IHC staining of mouse liver using 15393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15393-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS3 antibody (15393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

15393-1-AP bindet in WB, IP, IHC, IF, ELISA DHRS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Insekt
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHRS3 fusion protein Ag7589
• Vollständiger Name: dehydrogenase/reductase (SDR family) member 3
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC002730
• Gene symbol: DHRS3
• Gene ID (NCBI): 9249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).

Publikationen

Species	Application	Title
human	WB	J Clin Endocrinol Metab; Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells.; Authors - Kuroda Keiji K
mouse	IHC	FASEB J; The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development.; Authors - Billings Sara E SE
human,mouse	WB	J Biol Chem; Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice.; Authors - Olga V Belyaeva ; KD Validated
mouse	WB	FASEB J; Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage.; Authors - Junmei Zhou
human	WB	Mol Endocrinol; Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells.; Authors - Kuroda Keiji K
human	WB,IF	J Biol Chem; Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture.; Authors - Lee Seung-Ah SA