DHRS2 Polyklonaler Antikörper

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• KD/KO Validated

DHRS2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15735-1-AP

Synonyme

DHRS2, Dicarbonyl reductase HEP27, HEP27, Protein D, SDR25C1

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 15735-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15735-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15735-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15735-1-AP

  IP Result of anti-DHRS2 (IP:15735-1-AP, 4ug; Detection:15735-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human liver cancer using 15735-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15735-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, humanes Lebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

15735-1-AP bindet in WB, IP, IHC, ELISA DHRS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHRS2 fusion protein Ag8508
• Vollständiger Name: dehydrogenase/reductase (SDR family) member 2
• Berechnetes Molekulargewicht: 300 aa, 32 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC007339
• Gene symbol: DHRS2
• Gene ID (NCBI): 10202
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	Epigenetics; Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.; Authors - Yingyan Han ; KD Validated
human	WB	Oncol Rep; PMID: 31436301; Authors - Ji-Min Li ; KD Validated
human	WB	Cell Death Dis; REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.; Authors - Yunshang Chen
human	WB	Transl Oncol; STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression; Authors - Xiang Yang
human	IHC	Iran J Public Health; Auxiliary Diagnosis and Prognostic Value of Dehydrogenase/Reductase 2 (DHRS2) in Various Tumors; Authors - Zhongwu An