DHRS1 Polyklonaler Antikörper

DHRS1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16275-1-AP

Synonyme

DHRS1, SDR19C1

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Galerie der Validierungsdaten

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  WB analysis using 16275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16275-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16275-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16275-1-AP (DHRS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16275-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16275-1-AP (DHRS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 16275-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16275-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16275-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16275-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 16275-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DHRS1 antibody (16275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, humanes Lebergewebe, Maushodengewebe, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hautgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16275-1-AP bindet in WB, IHC, IF, ELISA DHRS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHRS1 fusion protein Ag9351
• Vollständiger Name: dehydrogenase/reductase (SDR family) member 1
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC014057
• Gene symbol: DHRS1
• Gene ID (NCBI): 115817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHRS1(Dehydrogenase/reductase SDR family member 1) is also named as LJ25430, MGC20204, DKFZp586I0523, SDR19C1, FLJ14250 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It is involved in the metabolism of a large variety of compounds, including steroid hormones, prostaglandins, retinoids, lipids and xenobiotics.Northern blot analysis detected DHRS1express in heart highest and in liver lowest.