DGKQ Polyklonaler Antikörper

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• KD/KO Validated

DGKQ Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17885-1-AP

Synonyme

DAG kinase theta, DAGK, DAGK4, DAGK7, DGK theta, DGKQ, Diacylglycerol kinase theta, Diglyceride kinase theta

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Galerie der Validierungsdaten

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  WB analysis using 17885-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17885-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17885-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervix using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Plazenta-Gewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

17885-1-AP bindet in WB, IHC, ELISA DGKQ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DGKQ fusion protein Ag11938
• Vollständiger Name: diacylglycerol kinase, theta 110kDa
• Berechnetes Molekulargewicht: 942 aa, 101 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC063801
• Gene symbol: DGKQ
• Gene ID (NCBI): 1609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Med Rep; Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.; Authors - Jingjing Zhang ; KO Validated
	WB	J Sci Food Agric; Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway; Authors - Guangyi Bai