DFNA5/GSDME Monoklonaler Antikörper
DFNA5/GSDME Monoklonal Antikörper für FC, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1D9C3
Kat-Nr. : 67731-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe |
| Erfolgreiche Detektion in FC | SH-SY5Y-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 3 publications below |
Produktinformation
67731-1-Ig bindet in WB, FC, ELISA DFNA5/GSDME und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | DFNA5/GSDME fusion protein Ag30514 |
| Vollständiger Name | deafness, autosomal dominant 5 |
| Berechnetes Molekulargewicht | 496 aa, 55 kDa |
| Beobachtetes Molekulargewicht | 50-55 kDa |
| GenBank-Zugangsnummer | BC019689 |
| Gene symbol | DFNA5 |
| Gene ID (NCBI) | 1687 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for DFNA5/GSDME antibody 67731-1-Ig | Protokoll herunterladen |
| FC protocol for DFNA5/GSDME antibody 67731-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Mitochondria-Targeting Pyroptosis Amplifier of Lonidamine-Modified Black Phosphorus Nanosheets for Glioblastoma Treatments | ||
Exp Neurol SNAP25 ameliorates postoperative cognitive dysfunction by facilitating PINK1-dependent mitophagy and impeding caspase-3/GSDME-dependent pyroptosis | ||
ACS Chem Neurosci Inhibition of PINK1-Mediated Mitophagy Contributes to Postoperative Cognitive Dysfunction through Activation of Caspase-3/GSDME-Dependent Pyroptosis |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH William (Verified Customer) (05-12-2021) | This antibody brightly stained bands of just over 50 kDa and just under 30 kDa, neither of which correspond to human GSDME, and it did so in samples from cells that do not express GSDME. The sample blots provided by ProteinTech also show bands that are too small to be GSDME. This does not appear to target GSDME.
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