DEPDC7 Polyklonaler Antikörper

DEPDC7 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26517-1-AP

Synonyme

DEP domain containing 7, DEPDC7, Protein TR2/D15, TR2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 26517-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 26517-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26517-1-AP (DEPDC7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26517-1-AP bindet in WB, IF,ELISA DEPDC7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DEPDC7 fusion protein Ag24094
• Vollständiger Name: DEP domain containing 7
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC030970
• Gene symbol: DEPDC7
• Gene ID (NCBI): 91614
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEPDC7 (DEP domain-containing protein 7), also known as protein TR2/D15, is a 511 amino acid protein belonging to the DEPDC7 family. DEPDC7 contains one DEP domain and is highly expressed in liver, with lower levels in kidney. DEPDC7 exists as two isoforms produced by alternative splicing events. The gene encoding DEPDC7 maps to human chromosome 11p13 and mouse chromosome 2 E2. With approximately 135 million base pairs and 1,400 genes, chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. DEPDC7 involves the regulation of small GTPase mediated signal transduction.