DENND1A Polyklonaler Antikörper

DENND1A Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 25658-1-AP

Synonyme

Connecdenn, DENN/MADD domain containing 1A, DENND1A, FAM31A, KIAA1608, Protein FAM31A, RP11 230L22.3

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 25658-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25658-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25658-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 25658-1-AP

  IP Result of anti-DENND1A (IP:25658-1-AP, 4ug; Detection:25658-1-AP 1:600) with K-562 cells lysate 3200ug.

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  IHC staining of human endometrial cancer using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 25658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 25658-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DENND1A antibody (25658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A2780-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25658-1-AP bindet in WB, IP, IHC, IF, ELISA DENND1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DENND1A fusion protein Ag22547
• Vollständiger Name: DENN/MADD domain containing 1A
• Berechnetes Molekulargewicht: 1009 aa, 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa, 64 kDa
• GenBank-Zugangsnummer: BC039703
• Gene symbol: DENND1A
• Gene ID (NCBI): 57706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DENND1A (DENN/MADD domain containing 1A), also known as Connecdenn,FAM31A or KIAA1608, is a 1,009 amino acid peripheral membrane protein. Recent studies has found DENND1A is strongly associated with PCOS (Polycystic ovary syndrome). DENND1A has several isoforms.This antibody(25658-1-AP) can recognize all the isoforms at 112 kDa, 52-64 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xin (Verified Customer) (03-15-2022)

There is a band close to 130 kD not like the predicted 110 kD around. It is not sure the identity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human postmortem brain lysate

DENND1A Antibody Western Blot validation (1:1000 dilution) in human postmortem brain lysate (Cat no:25658-1-AP)