DEK Monoklonaler Antikörper

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• KD/KO Validated

DEK Monoklonal Antikörper für FC (Intra), WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

5E3A6

Kat-Nr. : CL594-66194

Synonyme

D6S231E, DEK, DEK oncogene, Protein DEK

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Galerie der Validierungsdaten

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  WB analysis using CL594-66194

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66194 (DEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL594-66194

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DEK (CL594-66194, Clone:5E3A6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66194 bindet in WB, FC (Intra) DEK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DEK fusion protein Ag8225
• Vollständiger Name: DEK oncogene
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC035259
• Gene symbol: DEK
• Gene ID (NCBI): 7913
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011