DEK Monoklonaler Antikörper

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• KD/KO Validated

DEK Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

5E3A6

Kat-Nr. : 66194-1-Ig

Synonyme

D6S231E, DEK, DEK oncogene, Protein DEK

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Galerie der Validierungsdaten

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  WB analysis using 66194-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66194-1-Ig (DEK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66194-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66194-1-Ig (DEK antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66194-1-Ig bindet in WB, IHC, IF DEK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DEK fusion protein Ag8225
• Vollständiger Name: DEK oncogene
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC035259
• Gene symbol: DEK
• Gene ID (NCBI): 7913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB,IF	Biochem J; DEK terminates diapause by activation of quiescent cells in the crustacean Artemia.; Authors - Wen-Huan Jia ; KD Validated
	IHC	Mol Med Rep; Overexpression of DEK gene is correlated with poor prognosis in hepatocellular carcinoma.; Authors - Huo-Chun Yi