DDX46 Polyklonaler Antikörper

DDX46 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16927-1-AP

Synonyme

DDX46, DEAD box protein 46, KIAA0801, PRP5 homolog, PRPF5

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 16927-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16927-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16927-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 16927-1-AP

  IP Result of anti-DDX46 (IP:16927-1-AP, 4ug; Detection:16927-1-AP 1:1000) with NIH/3T3 cells lysate 2000ug.

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  IF Staining of HepG2 using 16927-1-AP

  Immunofluorescent analysis of HepG2 cells, using DDX46 antibody 16927-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

16927-1-AP bindet in WB, IP, IF, ELISA DDX46 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX46 fusion protein Ag10342
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
• Berechnetes Molekulargewicht: 1031 aa, 117 kDa
• Beobachtetes Molekulargewicht: 120-125 kDa
• GenBank-Zugangsnummer: BC012304
• Gene symbol: DDX46
• Gene ID (NCBI): 9879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.

Publikationen

Species	Application	Title
human	IF	Elife; Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.; Authors - Andrea Pawellek
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	IF	Sci Rep; Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.; Authors - Sunyuan Zhang
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu