DDX43 Monoklonaler Antikörper

DDX43 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3B2

Kat-Nr. : 68454-1-Ig

Synonyme

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, DKFZp434H2114, HAGE, Helical antigen

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Galerie der Validierungsdaten

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  WB analysis using 68454-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68454-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68454-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Calu-3 cells, DU 145-Zellen, HeLa-Zellen, Kaninchenhodengewebe, LNCaP-Zellen, NCCIT-Zellen, Rattenhodengewebe, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68454-1-Ig bindet in WB, ELISA DDX43 und zeigt Reaktivität mit human, Kaninchen, Ratten

• Getestete Reaktivität: human, Kaninchen, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX43 fusion protein Ag11484
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
• Berechnetes Molekulargewicht: 648 aa, 73 kDa
• Beobachtetes Molekulargewicht: 68-73 kDa
• GenBank-Zugangsnummer: BC066938
• Gene symbol: DDX43
• Gene ID (NCBI): 55510
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy.