DDX4,VASA Monoklonaler Antikörper

DDX4,VASA Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1F12C3

Kat-Nr. : 67147-2-Ig

Synonyme

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 67147-2-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 67147-2-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67147-2-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67147-2-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 67147-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 67147-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody (67147-2-Ig, Clone: 1F12C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, JAR-Zellen, NCCIT-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67147-2-Ig bindet in WB, IHC, IF, ELISA DDX4,VASA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX4,VASA fusion protein Ag11630
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Berechnetes Molekulargewicht: 690aa,76 kDa; 724aa,79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC047455
• Gene symbol: DDX4
• Gene ID (NCBI): 54514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Publikationen

Species	Application	Title
rat,mouse,human		iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min