DDX27 Polyklonaler Antikörper

DDX27 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 17087-1-AP

Synonyme

DDX27, DEAD box protein 27, HSPC259, PP3241, RHLP, Rrp3p

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17087-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17087-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 17087-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17087-1-AP (DDX27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

17087-1-AP bindet in WB, IF, ELISA DDX27 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX27 fusion protein Ag9426
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
• Berechnetes Molekulargewicht: 796 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC016060
• Gene symbol: DDX27
• Gene ID (NCBI): 55661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX27 is one of the DEAD box proteins, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DEAD box proteins are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division, and DDX27 is involved in the processing of 5.8S and 28S ribosomal RNAs.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.; Authors - Francesca Rossi
human	WB	Onco Targets Ther; DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells.; Authors - Chunxing Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wael (Verified Customer) (05-24-2023)

we detected the relative abundance of DDX27 in both the Nuclear and cytoplasmic fractions

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

DDX27 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:17087-1-AP)