DDX21 Monoklonaler Antikörper

DDX21 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B9C8

Kat-Nr. : 66925-1-Ig

Synonyme

DDX21, DEAD box protein 21, DKFZp686F21172, Gu alpha, GUA, GURDB, Nucleolar RNA helicase 2, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/GU, RH II/GuA

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Galerie der Validierungsdaten

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  WB analysis using 66925-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66925-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  IHC staining of human colon cancer using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66925-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66925-1-Ig (DDX21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66925-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human DDX21 (66925-1-Ig, Clone:1B9C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66925-1-Ig bindet in WB, IHC, IF, FC, ELISA DDX21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDX21 fusion protein Ag17850
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• Berechnetes Molekulargewicht: 783 aa, 87 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: BC008071
• Gene symbol: DDX21
• Gene ID (NCBI): 9188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011