DDX17 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDX17 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, PLA, IP, IHC, IF, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 19910-1-AP

Synonyme

DDX17, DEAD box protein 17, DEAD box protein p72, P72, RH70, RNA dependent helicase p72

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Galerie der Validierungsdaten

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19910-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19910-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19910-1-AP

  IP Result of anti-DDX17,P72 (IP:19910-1-AP, 4ug; Detection:19910-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 10x lens).

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  IF Staining of HeLa using 19910-1-AP

  Immunofluorescent analysis of Hela cells, using DDX17 antibody 19910-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293T-Zellen, HEK-293-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Produktinformation

19910-1-AP bindet in WB, PLA, IP, IHC, IF, CoIP, chIP, ELISA DDX17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX17 fusion protein Ag13723
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• Berechnetes Molekulargewicht: 729 aa, 80 kDa
• Beobachtetes Molekulargewicht: 72-80 kDa
• GenBank-Zugangsnummer: BC000595
• Gene symbol: DDX17
• Gene ID (NCBI): 10521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX17, also named as DEAD box protein p72 and DEAD box protein p82, is a 729 amino acid protein, which belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. DDX17 is widely expressed. Levels tend to increase during colon cancer progression, from very low in benign hyperplastic polyps to very high in tubular and villous adenomas. DDX17 as an RNA helicase unwinds RNA and alters RNA structures through ATP binding and hydrolysis. DDX17 is involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. It Regulates the alternative splicing of exons exhibiting specific features. identification and characterisation of p72, a novel human nuclear DEAD box protein, which shows a striking homology to p68. a predicted molecular mass of DDX17 is 72 kDa, but endogenous DDX17 migrates aberrantly at 79 kDa on SDS-PAGE (PMID: 8871553).

Publikationen

Species	Application	Title
human	CoIP,chIP	Nat Commun; Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1.; Authors - Lamya Ben Ameur
human	IP	Nucleic Acids Res; The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation.; Authors - Marie-Pierre Lambert
human	WB,IP	Nucleic Acids Res; RNA helicase-dependent gene looping impacts messenger RNA processing; Authors - Sophie Terrone ; KD Validated
human	WB	PLoS Pathog; Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production.; Authors - Hélène Chabrolles
human	IF	J Biol Chem; RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression.; Authors - Christine Xing'er Koo
human	PLA	iScience; Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells; Authors - Nam-Kyung Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

N (Verified Customer) (01-28-2023)

Perfect to detect double bands of DDX17.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

DDX17 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:19910-1-AP)