DDRGK1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDRGK1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 21445-1-AP

Synonyme

C20orf116, DDRGK domain containing 1, DDRGK1, UFBP1

---

Galerie der Validierungsdaten

• 

  WB analysis using 21445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21445-1-AP (DDRGK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21445-1-AP

  Immunofluorescent analysis of HepG2 cells using 21445-1-AP (DDRGK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe, Mauslungengewebe, PC-3-Zellen, Rattenlebergewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IHC: See 8 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

21445-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA DDRGK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDRGK1 fusion protein Ag13742
• Vollständiger Name: DDRGK domain containing 1
• Berechnetes Molekulargewicht: 314 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-46 kDa
• GenBank-Zugangsnummer: BC000643
• Gene symbol: DDRGK1
• Gene ID (NCBI): 65992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

Publikationen

Species	Application	Title
human	WB	Cell; A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.; Authors - Jin Rui Liang ; KD Validated
human	IHC	Nat Cell Biol; UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination.; Authors - Jiang Liu
		Cell Res; UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis.; Authors - Lihui Wang
human	WB,IHC,IF	Adv Sci (Weinh); DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination; Authors - Xin Wang ; KO Validated
human	WB	Sci Adv; Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control; Authors - Ryosuke Ishimura ; KO Validated
human	WB	Nat Commun; A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1α stability.; Authors - Jiang Liu ; KD Validated