DDOST Polyklonaler Antikörper

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• KD/KO Validated

DDOST Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 14916-1-AP

Synonyme

AGE R1, DDOST, DDOST 48 kDa subunit, KIAA0115, OK/SW cl.45, OST, OST48, WBP1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14916-1-AP

  WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected HeLa cells.

• 

  WB analysis of A549 using 14916-1-AP

  WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected A549 cells.

• 

  WB analysis using 14916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14916-1-AP (DDOST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat small intestine using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse pancreas using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse pancreas using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen, A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maus-Pankreasgewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14916-1-AP bindet in WB, IHC, CoIP, ELISA DDOST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDOST fusion protein Ag6727
• Vollständiger Name: dolichyl-diphosphooligosaccharide-protein glycosyltransferase
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC002594
• Gene symbol: DDOST
• Gene ID (NCBI): 1650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
mouse	WB,CoIP	Res Sq; Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction; Authors - Hossein Ardehali
human	IHC	IET Syst Biol; An immune-related multi-omics analysis of dolichyl-diphosphooligosaccharide protein glycosyltransferase in glioma: Prognostic value exploration and competitive endogenous RNA network identification; Authors - Jie Liu
human	IHC	J Hepatocell Carcinoma; Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma; Authors - Shusheng Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicole (Verified Customer) (04-28-2022)

80µl RIPA Hela cell extract: Nitrocellulose: no signal PVDF: weak signal, double band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela