DYKDDDDK tag Rekombinanter Antikörper
DYKDDDDK tag Rekombinant Antikörper für IF, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
rekombinanten Protein und mehr (3)
Anwendung
RIP, IP, IF, FC, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
4K14
Kat-Nr. : 80010-1-RR
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche IP | Transfizierte HEK-293-Zellen |
| Erfolgreiche Detektion in IF | Transfizierte HEK-293-Zellen, Transfizierte HEK-293-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunfluoreszenz (IF) | IF : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| IF | See 8 publications below |
| IP | See 24 publications below |
| FC | See 1 publications below |
| CoIP | See 8 publications below |
| RIP | See 1 publications below |
Produktinformation
80010-1-RR bindet in RIP, IP, IF, FC, CoIP, ELISA DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein
| Getestete Reaktivität | rekombinanten Protein |
| In Publikationen genannte Reaktivität | human, Affe, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Rekombinant |
| Typ | Antikörper |
| Immunogen | DYKDDDDK tag fusion protein Ag2329 |
| Vollständiger Name | DYKDDDDK tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| IF protocol for DYKDDDDK tag antibody 80010-1-RR | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
PLoS Biol CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner | ||
Cancer Lett N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression. | ||
Cancer Lett ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xin (Verified Customer) (04-17-2022) | It is good to detect flag-tagged protein expressed in hek293 cells, although efficiency is not so high as 20543-1-AP.
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