DDAH2 Polyklonaler Antikörper

DDAH2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14966-1-AP

Synonyme

DDAH, DDAH 2, DDAH2, DDAHII, Dimethylargininase 2, G6a, NG30, Protein G6a, S phase protein

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Galerie der Validierungsdaten

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  WB analysis using 14966-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14966-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14966-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 14966-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 14966-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14966-1-AP

  IP Result of anti-DDAH2 (IP:14966-1-AP, 3ug; Detection:14966-1-AP 1:500) with COLO 320 cells lysate 2000ug.

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  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

14966-1-AP bindet in WB, IP, IHC, ELISA DDAH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDAH2 fusion protein Ag6831
• Vollständiger Name: dimethylarginine dimethylaminohydrolase 2
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001435
• Gene symbol: DDAH2
• Gene ID (NCBI): 23564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).

Publikationen

Species	Application	Title
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang
		Clin Sci (Lond); DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase.; Authors - Heng-Huei Lin
human	WB	FASEB J; Neuropilin-1 maintains dimethylarginine dimethylaminohydrolase 1 expression in endothelial cells, and contributes to protection from angiotensin II-induced hypertension.; Authors - Ying Wang
human	WB	iScience; Dissecting VEGF-induced acute versus chronic vascular hyperpermeability: Essential roles of dimethylarginine dimethylaminohydrolase-1.; Authors - Ying Wang
rat	WB,IHC	PLoS One; Involvement of DDAH/ADMA/NOS/cGMP and COX-2/PTGIS/cAMP Pathways in Human Tissue Kallikrein 1 Protecting Erectile Function in Aged Rats.; Authors - Kai Cui
mouse,human	WB	Nat Commun; A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase; Authors - Vinitha N Ragavan ; KO Validated