DCUN1D5 Polyklonaler Antikörper

DCUN1D5 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14810-1-AP

Synonyme

DCN1 like protein 5, DCUN1D5

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 14810-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14810-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 14810-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14810-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 14810-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14810-1-AP

  IP Result of anti-DCUN1D5 (IP:14810-1-AP, 3ug; Detection:14810-1-AP 1:800) with HeLa cells lysate 1840ug.

• 

  IF Staining of HeLa using 14810-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14810-1-AP (DCUN1D5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Maushirngewebe, Rattenmilzgewebe, U-937-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

14810-1-AP bindet in WB, IP, IHC, IF,ELISA DCUN1D5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCUN1D5 fusion protein Ag6534
• Vollständiger Name: DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae)
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC004169
• Gene symbol: DCUN1D5
• Gene ID (NCBI): 84259
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Conjugation of Nedd8 to a cullin protein, termed neddylation, is an evolutionarily conserved process that functions to activate the cullin-RING family E3 ubiquitin ligases, leading to increased proteasomal degradation of a wide range of substrate proteins. Cellular neddylation requires the action of DCN1 (Defective in cullin neddylation protein 1), which, in humans, consists of five homologues designated as DCNL1-5. DCUN1D5 gene encodes the 28 kDa DCUN1 domain-containing protein 5 containing a C-terminal potentiating neddylation domain. The cellular function of DCUN1D5 needs to be explored, recently, a mono-ubiquitination-mediated mechanism that governs nuclear-cytoplasmic trafficking of hDCNL1, was reported to regulate hDCNL1-dependent activation of the cullin-RING E3 ubiquitin ligases in selected cellular compartments.

Publikationen

Species	Application	Title
human	WB	Elife; SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function.; Authors - Kate E Coleman
human	WB,IHC	Asian Pac J Cancer Prev; In vitro biological characterization of DCUN1D5 in DNA damage response.; Authors - Guo Wei W