DCTN6 Polyklonaler Antikörper

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• KD/KO Validated

DCTN6 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16947-1-AP

Synonyme

DCTN6, dynactin 6, Dynactin subunit 6, Dynactin subunit p27, Protein WS 3, WS 3, WS3

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Galerie der Validierungsdaten

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  WB analysis using 16947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16947-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 16947-1-AP

  IP Result of anti-DCTN6 (IP:16947-1-AP, 4ug; Detection:16947-1-AP 1:500) with mouse testis tissue lysate 4800ug.

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  IHC staining of mouse testis using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maushodengewebe, Mauslungengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:600-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

16947-1-AP bindet in WB, IP, IHC, IF, ELISA DCTN6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCTN6 fusion protein Ag10561
• Vollständiger Name: dynactin 6
• Berechnetes Molekulargewicht: 190 aa, 21 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC013175
• Gene symbol: DCTN6
• Gene ID (NCBI): 10671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
Monkey	WB	EMBO J; Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.; Authors - Yeh Ting-Yu TY ; KD Validated
human	WB	Curr Biol; An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.; Authors - Florencia di Pietro
human	WB	Mol Biol Cell; Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles.; Authors - Samantha L Cavolo
mouse	WB,IF,IP	Neurobiol Aging; Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.; Authors - Md Golam Sharoar ; KD Validated