DCLK1 Monoklonaler Antikörper

DCLK1 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C12C1

Kat-Nr. : 68234-1-Ig

Synonyme

DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369

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Galerie der Validierungsdaten

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  WB analysis using 68234-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of fetal human brain using 68234-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of mouse brain using 68234-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68234-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 68234-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (68234-1-Ig, Clone: 3C12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Neuro-2a using 68234-1-Ig

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human DCLK1 (68234-1-Ig, Clone:3C12C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, fetales humanes Hirngewebe, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe
• Erfolgreiche Detektion in FC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68234-1-Ig bindet in WB, IHC, IF, FC, ELISA DCLK1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DCLK1 fusion protein Ag17110
• Vollständiger Name: doublecortin-like kinase 1
• Berechnetes Molekulargewicht: 729 aa, 81 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 82 kDa
• GenBank-Zugangsnummer: BC152456
• Gene symbol: DCLK1
• Gene ID (NCBI): 9201
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.