DCK Polyklonaler Antikörper

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• KD/KO Validated

DCK Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17758-1-AP

Synonyme

DCK, deoxycytidine kinase

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Galerie der Validierungsdaten

• 

  WB analysis using 17758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17758-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17758-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17758-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17758-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

17758-1-AP bindet in WB, ELISA DCK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCK fusion protein Ag12123
• Vollständiger Name: deoxycytidine kinase
• Berechnetes Molekulargewicht: 260 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC103764
• Gene symbol: DCK
• Gene ID (NCBI): 1633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443).

Publikationen

Species	Application	Title
human	WB	Oncogene; KNSTRN promotes tumorigenesis and gemcitabine resistance by activating AKT in bladder cancer.; Authors - Yaoyi Xiong
human	WB	Am J Cancer Res; PMID: 32368394; Authors - Zeng Ye
human	WB	Int J Cancer; Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.; Authors - Nao Yoshida-Sakai
human	WB	Front Oncol; m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.; Authors - Congjun Zhang
human	WB	Invest New Drugs; Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma.; Authors - Irene Dell'Anno
human	WB	Sci Rep; Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia.; Authors - Susan K Rathe ; KO Validated