DBR1 Monoklonaler Antikörper

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• KD/KO Validated

DBR1 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B5A7

Kat-Nr. : 66951-1-Ig

Synonyme

DBR1, Lariat debranching enzyme

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66951-1-Ig

  WB result of DBR1 antibody (66951-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells.

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  WB analysis using 66951-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66951-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66951-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66951-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66951-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66951-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 66951-1-Ig

  IP result of anti-DBR1(IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with HeLa cells lysate 1560 ug.

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  IP experiment of mouse brain using 66951-1-Ig

  IP result of anti-DBR1(IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 66951-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66951-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66951-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DBR1 antibody (66951-1-Ig, Clone: 3B5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, fetales humanes Hirngewebe, Hausschwein-Hirngewebe, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66951-1-Ig bindet in WB, IP, IHC, IF, ELISA DBR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DBR1 fusion protein Ag8830
• Vollständiger Name: debranching enzyme homolog 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 544 aa, 62 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC009472
• Gene symbol: DBR1
• Gene ID (NCBI): 51163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.