DBR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DBR1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (2)

Kat-Nr. : 16019-1-AP

Synonyme

DBR1, Lariat debranching enzyme

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 16019-1-AP

  WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells.

• 

  WB analysis of human brain using 16019-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16019-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16019-1-AP

  IP result of anti-DBR1(IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:800) with HeLa cells lysate 1560 ug.

• 

  IP experiment of mouse brain using 16019-1-AP

  IP result of anti-DBR1(IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:500) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human placenta using 16019-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 16019-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16019-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DBR1 antibody (16019-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: Maushirngewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

16019-1-AP bindet in WB, IP, IHC, IF, ELISA DBR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DBR1 fusion protein Ag8830
• Vollständiger Name: debranching enzyme homolog 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 544 aa, 62 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC009472
• Gene symbol: DBR1
• Gene ID (NCBI): 51163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nat Genet; Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.; Authors - Armakola Maria M ; KD Validated
human	WB	Cell; Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.; Authors - Shen-Ying Zhang
human	WB	Mol Cell Proteomics; Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.; Authors - Syred Heather M HM
human	IF	PLoS One; Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus.; Authors - Anne Hett
human	WB, IHC	Int J Mol Sci; Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.; Authors - So Masaki
human	IHC	Technol Cancer Res Treat; The Role of DBR1 as a Candidate Prognosis Biomarker in Esophageal Squamous Cell Carcinoma.; Authors - Xiaoqin Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Marta (Verified Customer) (02-18-2020)

Single band in the WB at the expected size, incubated overnight at 4C with a 1/1200 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1200
• Cell Tissue Type: PBMC, B-EBV cells

DBR1 Antibody Western Blot, validation (1/1200 dilution) in PBMC, B-EBV cells (Cat no:16019-1-AP)

FH

Aiswarya (Verified Customer) (12-23-2019)

The Dbr1 antibody was used to check knock down of Dbr1 and worked well. Additional non specific bands come up but do not interfere in the specific size range.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1, HEK293