DAAM2 Polyklonaler Antikörper

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• KD/KO Validated

DAAM2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 25206-1-AP

Synonyme

DAAM2, KIAA0381

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 25206-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 25206-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 25206-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DAAM2 antibody (25206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

25206-1-AP bindet in WB, IHC, IF, ELISA DAAM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAAM2 fusion protein Ag18955
• Vollständiger Name: dishevelled associated activator of morphogenesis 2
• Berechnetes Molekulargewicht: 1068 aa, 123 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC128388
• Gene symbol: DAAM2
• Gene ID (NCBI): 23500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Front Mol Biosci; Dishevelled-Associated Activator of Morphogenesis 2 (DAAM2) Predicts the Immuno-Hot Phenotype in Pancreatic Adenocarcinoma.; Authors - Qinglin Zhang
human	IHC,WB	Clin Transl Oncol; The prognostic value of DAAM2 in lower grade glioma, liver cancer, and breast cancer; Authors - Zeying Li ; KD Validated
mouse	IHC	Clin Immunol; Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy; Authors - Jie Mei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erithelgi (Verified Customer) (11-11-2019)

The antibody was verified in an siRNA mediated knockdown

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS osteosarcoma cells