Cytokeratin 7 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 7 Monoklonal Antikörper für FC(Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF, FC(Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

2E1G5

Kat-Nr. : CL555-66483

Synonyme

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Galerie der Validierungsdaten

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  IF Staining of human prostate cancer using CL555-66483

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

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  IF Staining of human prostate cancer using CL555-66483

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-66483

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Cytokeratin 7 (CL555-66483, Clone:2E1G5) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Prostatakarzinomgewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66483 bindet in IF, FC(Intra) Cytokeratin 7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Vollständiger Name: keratin 7
• Berechnetes Molekulargewicht: 469 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC002700
• Gene symbol: KRT7
• Gene ID (NCBI): 3855
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C