Cytokeratin 7 Monoklonaler Antikörper

Cytokeratin 7 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G2H1

Kat-Nr. : 68296-1-Ig

Synonyme

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Galerie der Validierungsdaten

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  WB analysis using 68296-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68296-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EC109-Zellen, A431-Zellen, A549-Zellen, BxPC-3-Zellen, HaCaT-Zellen, HeLa-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:4150-1:16600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68296-1-Ig bindet in WB, IHC, ELISA Cytokeratin 7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Vollständiger Name: keratin 7
• Berechnetes Molekulargewicht: 469 aa, 51 kDa
• GenBank-Zugangsnummer: BC002700
• Gene symbol: KRT7
• Gene ID (NCBI): 3855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is a marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.