Cytokeratin 19 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 19 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

3G1E4

Kat-Nr. : CL555-60187

Synonyme

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Galerie der Validierungsdaten

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  IF Staining of human breast cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  IF Staining of human oesophagus cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

• 

  IF Staining of human oesophagus cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-60187

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 19 (CL555-60187, Clone:3G1E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-60187 bindet in IF, FC (Intra) Cytokeratin 19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag7407
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC002539
• Gene symbol: KRT19
• Gene ID (NCBI): 3880
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C