Cytokeratin 18 Monoklonaler Antikörper

Cytokeratin 18 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Hund

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G11C4

Kat-Nr. : CL488-66187

Synonyme

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

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  IF Staining of MDCK using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using CL488-66187

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Cytokeratin 18 (CL488-66187, Clone:1G11C4) (green), and 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen, MDCK-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66187 bindet in IF, FC (Intra) Cytokeratin 18 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: KRT18
• Gene ID (NCBI): 3875
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.